A. RajaSekhar Reddy , C. V. Naidu

Abstract

Azadirachta indica is a typical tropical/sub-tropical plant were used for micropropagation and generation of plantlets from callus cultures. Induction of somaclonal variability in the existing germplasm was another aim. Callus was initiated from young leaves on MS medium supplemented with different concentrations of BA (2 mg/l) and 2,4-D (0.5 mg/l). Organogenesis was achieved by transferring callus on MS medium supplemented with TDZ (3.0 mg/l) and adenine (1.0 mg/l) getting a very high frequency of shoot regeneration 81.3 per cent. Well-grown shoots ischolar_mained easily on MS-half supplemented with IBA +IAA (2.0+0.5 mg/l) and 80-90% ischolar_maining was achieved. Plantlets got hardened by keeping them in hardening unit for few days. Hardened plants established very well in the nursery. Though originated from callus, these plants did not show any morphological variations and were similar to their parent donar plant. Histological study revealed that regeneration took place through denovo merestemoid formation.

Keywords

Callus Induction, Regeneration, In-vitro, Azadirachta indica

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A. RajaSekhar Reddy , P. S. Rao , J. V. Sreenivasa Rao

Abstract

The effect of seasonal variation on ischolar_maining potential of Neem by using mid-cuttings, hardwood cuttings and epicormic shoots was studied at Biotechnoloty Research Centre (A.P. Forest Department), Triupati. The plant harmones such as IBA, IAA, 2.4-D were used alone or in combinations of different concentrations viz., 1000, 2000, 3000 ppm tested in both summer and winter seasons. Roots were observed in all types of branch cuttings after 30 days. During summer (April-May) 95 per cent of ischolar_maining was observed in epicormic shoots when treated with 1000 ppm IBA. However, in mid- and hardwood cuttings only 50% ischolar_maining was observed. During winter season (Nov.-Dec.) the epicormic shoots resulted in 50 per cent of ischolar_maining only. The individual concentrations showed better results as compared to combinations. Higher percentage of ischolar_maining was observed in summer compared to winter season.

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